Our recombinant human FGF-2 growth factor is produced in non-GMO insects, specifically in Trichoplusia ni (cabbage moth) with the Baculovirus Expression Vector System (BEVS).
Recombinant FGF-2 Human
For research use only
Basic Fibroblast Growth Factor, known as FGF basic or FGF-2, is a member of the fibroblast growth factor FGF family.
FGF-2 is commonly used to support the maintenance of human embryonic stem cells and the proliferation and differentiation of induced pluripotent and mesenchymal stem cells. It’s a component used in tissue engineering constructs and cellular agriculture.
Source
Sequence
Human FGF-2 recombinant protein is a monomeric peptide of 155 amino acid residues.
MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Formulation
Phosphate 20mM; NaCl 500mM; Tween20 0.05%; pH 7.4
Formulation
Phosphate 20mM; NaCl 500mM; Tween20 0.05%; pH 7.4
Purity
> 80% measured by SDS page, and resolved under reduced (R) and non-reduced (NR) conditions. Higher purity available upon request.
Biological Activity
ED50 <5 ng/ml in cellular/luciferase assays determined by the FGF2 dose dependent activation of the Col1 A2 gene promoter in NIH-3T3 cells. Determine the optimal concentration for each specific application using an initial dose response assay.
Molecular Weight
18.5 kDa
Format
Liquid Frozen, contact us for lyophilized product.
Downloads
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